AGCT is for Ambiguity, Graphs, and Carpel Tunnel

The sequences came in yesterday, but I didn't get a chance to look at them until today. After three hours of clicking, dragging, and editing, I had a compilation of all the DNA fragments we have sequenced so far.

The changes that I previously believed to be mutations may turn out to be something else entirely. After I assembled the contig file, I found that the differences in the DNA sequences were too numerous and too consistent to be DNA mutations. It was suggested to me that the plants are heterozygous, meaning that several sites may regularly have one of two bases. If this is the case, the sequencing part of my project will conclude with a recommendation that further research be done in this area. It is of course possible that the mutations are in a different part of the gene. This is quite believable, given that the BRI-1 gene is about 2000 bases long, and we have only sequenced about 1200 bases.

That being said, I doubt that I will have many problems writing a full-sized paper for my project, but it just won't be as conclusive as it could be.