So, the sequences came in today. I loaded them up on the Vector NTI program and did some "trimming" to get rid of some of the garbled, ambiguous sections. Frans says that the sequences look fine, but we will have to have more information in order to come to a conclusion about where the individual mutations are. (I am looking for one base-pair changes in sequences that will end up being 500-2000 bases long.)
I started running some more PCR reactions. I was initially keeping track of all the PCRs I did, but I stopped somewhere around 30. Once these PCRs are finished, we will hopefully be able to purify them and send them in for sequencing.
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