Moving Forward

So this is the week in which I should be getting my results. It's actually really exciting! I am working somewhere close to over time. Yesterday Mario told me it would be great to have my help finishing the work left by my undergrad friend, but he would be too busy until after 4, so I worked from 4-9 pm. It was a basic PCR run, but we needed to have the gel running by the time we called it quits. I have to admit it was strange for it to be sunny when I went in, and then full-on night when I got out.

My prediction for my week is that I will be running RT-PCR on samples of Heterozygous Mop3 and Homozygous Mop3 from which I will be able to compare gels and determine the differences in regulation. This process will take a few days because we must extract RNA and then carry out the Reverse Transcriptase part of the RT-PCR.