This week, I hope to continue to make progress in completely sequencing three introns in the attractin gene. I arrived to the laboratory this morning and was pleased to find new DNA sequences in my folder on the computer that I sent for sequencing last week. All of the samples appear to have been sequenced successfully, and I am eager to finish cleaning the data in search of heterozygotes (individuals with two versions of the same gene). From the data that I have received and cleaned, both intron 4 and 23 show two regions of heterozygosity. I also completed cleaning all of the sequences for intron 17 today (intron 17 was the first region I began sequencing)!
Additionally, I ran through a set of PCR reactions, but must repeat these reactions tomorrow in search of the source of error; possibly, the annealing temperature is not appropriate for the reaction, as it has caused issues with this intron in previous trials. Today proved to me once again how the field of research is truly a field of problem solving, as well as discovery.
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