Friday, March 12, 2010

More waiting...

Yesterday wasn't too eventful. I ran PCR again, and right now I'm waiting for the gel to run.

I've started applying brassinosteroids to my dwarfed Oleracea plants every day that I'm in the lab. I use a pipette to place 10 microliters on the point where new leaves are forming. From here, two things could happen. In one case, the plants might overcome their dwarfism and grow to the full height of an unmutated, wild type Oleracea. If this happens, the plants are brassinosteroid-sensitive, and that will tell us that the receptors are functioning normally. If nothing happens, we can conclude that the plants are brassinosteroid-insensitive.

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